Detección de antígeno p27 de ViLeF empleando el método de microELISA vs dos test inmuncromatográficos comercializados en la ciudad de Guayaquil.

Abstract

This investigation is comparative, non-experimental, that was carried out from the collection of cat’s serums within the months of November to December 2022 at the Veterinary Clinical Laboratory Diagnovet located in Guayaquil city, province of Guayas. The sample size was 40 cat’s serum that were sent to the laboratory previously mentioned for the detection of antigen p27 of feline leukemia virus (FeLV) using the micro-ELISA method, which were divided into 30 positives and 10 negatives. The investigation had three phases: First, the collection of cat’s serums; second, the same serum was used too on the two serological rapid tests that are commonly worn in vet consults, to evaluate the level of agreement and sensibility. The third phase consisted on tabulation of the data obtained and statistical analysis. It was possible to demonstrate statistically through kappa range, that both serological rapid tests are very good compared with micro-ELISA method as the gold standard technique, so it’s concluded that both tests are reliable and can be worn as tool for a rapid diagnose.