Análisis microbiológico de la desinfección del dique de goma usando cuatro protocolos. Estudio in vitro.

Abstract

INTRODUCTION: It is known that the use of contaminated materials can introduce microorganisms to the root canals during endodontic treatment. A prior requirement is the use of a sterile operative field and aseptic surgical techniques, since in this way the risk factors that may contaminate the root canal systems are diminished. OBJECTIVES: To evaluate the effectiveness of four different disinfection protocols in rubber dams. MATERIALS AND METHODS: In the following investigation, an in vitro experimental study of a descriptive and observational type, was carried out at the Centro de Investigación Microbiologico ¨Dr. Henry Parra¨, during the months of November to February of 2018. This experimental study compared the level of disinfection of the four types of protocols that were used in the infected rubber dams. RESULTS: It was found in the present study that the disinfection of the rubber dam can be obtained 100% with the use of 5.25% Sodium Hypochlorite, 0.12% Chlorhexidine, and 2% Chlorhexidine for 5 minutes. With the protocol from Möller, a disinfection of 33.3% can be obtained during 15 minutes. DISCUSSION: With the research carried out at the Centro de Investigación Microbiológico, the results obtained may lead us to consider that the Möller protocol is not as reliable during the disinfection of the rubber dam. Due to an exothermic reaction that is presented to the contact of the water oxygenate and iodine, and being a procedure that requires a lot of time, the Möller protocol failed to compete with the protocols of 5.25% Sodium Hypochlorite, 0.12%, Chlorhexidine, and 2% Chlorhexidine, which managed to disinfect 100% of the dams in a less amount of time. CONCLUSION: Based on the experiment carried out, it can be concluded that for disinfection of rubber dams, any of the protocols of 5.25% Sodium Hypochlorite, 0.12% Chlorhexidine and 2% Chlorhexidine can be used.