Análisis bacteriológico del instrumental quirúrgico post-esterilización de la clínica de cirugía UCSG mediante tinción de Gram.

Abstract

Introduction: Dental extractions are the most delicate procedures that have contact with the tissue of oral cavity, and it is necessary to have a sterile instrument to avoid any type of direct infection to the patient, in which in some part of the sterilization process would be affected. Objective: The objective of this work is to analyze the influence of the cleaning process, disinfection, sterilization and residual bacterial in surgical instruments. Materials and Methods: An in vitro, descriptive and cross-sectional study was carried out. The study population consisted of students of surgery I, II, III, IV of the dental clinic of the Catholic University of Santiago de Guayaquil. 100 samples were taken with swabs, sterile in a random way, it has been made a rubb in a Pettri Box with blood agar and incubated for 48 hours. Postincubation staining was performed on slides with Gram stain. Results: It was shown that 37% of the sample did not appear any type of microorganism, while the remaining 63% was microorganisms, after the incubation of the samples in blood agar, of which 31 samples (49%) are Gram- and 32 of the samples (51%) are Gram +; By means of the survey that was carried out to the students of the kit of instruments, it was possible to analyze the technique of washing, disinfection, type of packaging and sterilization.