Detección de SARS-CoV-2 en saliva y líquido crevicular de pacientes de la clínica odontológica (Semestre B-2022).

Abstract

Background: The oral cavity has been defined as one of the most important entrance points for the virus; its location in confluence with the respiratory tract, as well as saliva drainage, predisposes it to be a focus of great interest in regards to SARS-CoV-2. Oral bacteria, periodontal pathogens and, in general, periodontal disease have been associated with respiratory conditions. The dental environment is in constantly exposition due to direct contact with the oral cavity and its fluids. Aim: Detect SARS-CoV-2 in saliva and crevicular fluid of patients at the dental clinic. Methods: 50 patients who attended to Dental Clinic in a period of time from November 2022 to January 2023, in range of age between 16 to 75 years old, with diagnoses of gingivitis (40%) and periodontitis (60%) were chosen. Saliva and crevicular fluid samples were collected to be subjected to extraction and purification of viral nucleic acids that were subjected to amplification by reverse transcription-polymerase chain reaction (RT-PCR). Results: The frequency of SARS-CoV-2 in the crevicular fluid and saliva samples are equal in values of 55.6% for Gingivitis cases, and 44.4% for Periodontitis cases. Conclusion: It was verified that both crevicular fluid and saliva are fluids that allow the noninvasive diagnosis of SARS-CoV-2, there will be needed more studies in order to determine if GCF samples are equivalent to saliva samples for the diagnosis of SARS-CoV-2.