Diagnóstico de Hepatozoon spp mediante la Técnica Buffy Coat en caninos en una clínica veterinaria del cantón Daule.

Abstract

Hepatozoon spp., is a protozoon that can infect various animals, including canines, these can be infected by ingesting the brown dog tick Rhipicephalus sanguineous, this is great importance due to the huge epidemiological impact it generates on health. The present research work had a quantitative design, non-experimental and correlational type. The results were obtained by means of the Buffy Coat Technique, were collected from 100 blood samples from canines with presumptive signs of Hepatozoon spp at the VetMav veterinary clinic, in Daule. It was possible to observe infections and coinfections with the 100x objective, with 68 canines being positive for Ehrlichia spp, 18 canines showing Ehrlichia and Babesia coinfection. In the statistical analysis with chisquare, it is expressed that there is no significance between the clinical signs and the presence of Hepatozoon spp, (p≥ 0.05). In microscopy with the Buffy Coat Technique, one gamont per slide was found in neutrophils and monocytes with an irregular shape, not oval, which does not coincide with the characteristics associated with the protozoan. In conclusion, the presence of Hepatozoon spp was not determined in the months of May and June, however the transfer of animals from other countries and continents is a probability of its spread.