Viabilidad de las células del ligamento periodontal usando solución genérica de Hank. Estudio in vitro.

Abstract

Introduction: An avulsed tooth can be reimplanted without complications if reimplantation occurs within the first 20 minutes when stored in a dry medium, this time can increase from 1 to 3 hours if the tooth is placed in a suitable transport solution until the time of reimplantation. Aim: To reproduce an exclusive transport solution for cell viability of avulsed tooth in Ecuador. Materials and methods: 62 recently extracted teeth were used and stored in different storage media: Milk, Save a Tooth, Generic Hank Solution (SGH), Water, Saline and Gatorade at different storage times: 30, 60 and 120 minutes, a scraping of the apical third with a scalpel was performed on a plate and then stained and analyzed under microscopy. Results: The highest values of cell viability occurred at the temperature of 9.5ºC, (75% SGH, 70% Saline, 70% Water, 60% Milk, 60% Gatorade). However at a temperature of 23.5 ° C, the only representative result was the SGH with 60% cell viability, while the other storage media did not present representative ranges of cell viability (Saline 45%, Water 40%, Milk 25%, and Gatorade 45%). Conclusions: The SGH presented superior results in cellular viability of the periodontal ligament at a temperature of 23.5ºC (room temperature) compared to the other storage media.